I'm just putting this up for anyone who has had any difficluty exporting anime studio files using the export / swf movie option from the export settings. There seems to be some illegal use of splines that can lead to anime ignoring/not rendering any layers where illegal spline operations have taken place on.
Illegal Spline

in this example, due to the spline curvature, the a single spline path overlaps itself, creating an illegal loop.
Legal Spline

in this example, the curvature has been corrected, so the path does not cross itself.
Sure this is stuff that's blindly obvious to most but it tripped me up!
All the Best,
Matthew